hutu80 cells  (ATCC)

Journal: bioRxiv

Article Title: Human enterochromaffin cells and apical-out intestinal organoids as models for human sapovirus infection

doi: 10.64898/2026.03.06.710023

Figure Lengend Snippet: Phylogenetic tree of sapovirus samples (n = 27), color coded by genotype, and reference strains. The tree was constructed with Maximun likelihood algorithm using Kimura 2-parameter model (gamma distributed with invariant sites) and 1000 bootstrap replicates. Scale bar for branch lengths shows number of substitutions per site. Samples F20, F21, F30 and F31 were excluded in the analysis due to incomplete sequences. Reference strains: GII.1/Hu/IND/2021/NICED-BCH-12967 (GenBank: LC851686 ), GII.1/Hu/JPN/2013/Mie-493 (GenBank: LC816490 ), GII.1/Hu/BGD/2012/475050 (GenBank: MW088928 ), GII.1/Hu/UK/1998/Bristol (GenBank: AJ249939 ), GII.3/Hu/JPN/2023/20220111 (GenBank: LC848995 ), GII.3/Hu/JPN/2023/S22-415 (GenBank: LC849000 ), GII.3/Hu/JPN/2019/Mie-021 (GenBank: LC816544 ), GII.3/Hu/JPN/2017/Mie-835 (GenBank: LC816541 ), GI.1/Hu/US/2014/ Portland3639 (Genbank: MG012399 ), GI.1/Hu/IND/2022/NICED-BCH-13235 (GenBank: LC851695 ), GI.1/Hu/JPN/2011/EH-70 (GenBank: LC504316 ), GI.1/Hu/JPN/2023/V231401 (GenBank: LC848977 ), GI.1/Hu/JPN/1982/MT-2010 (GenBank: HM002617 ), GII.1/Hu/US/2015/Nashville9343 (GenBank: MG012444 ), GI.1/Hu/GER/pJG-Sap01/Dresden (GenBank: AY694184 ), GI.3/Hu/SWE/1997/318/Stockholm (GenBank: AF194182 ), GII.3/Hu/US/2015/Nashville9354 (GenBank: MG012419 ), GI.1/Hu/UK/1993/Manchester (GenBank: X86560 ). Abbreviations: Hu: Human, IND: India, JPN: Japan, US: United States, BGD: Bangladesh, GER: Germany, SWE: Sweden, UK: United Kingdom. B) Viral load (genome copies/gram of feces) and genotype of 31 sapovirus positive samples. Each colored square corresponds to one sample, and the median viral load is shown for each genotype. p-value, determined by Kruskal-Wallis test, is displayed. C) Detection of CD36 by gene expresison analysis and immunofluorescence staining in GOT1 and HuTu80. Gene expression of CD36 is displayed as inverted ΔCt value (1/ ΔCt), with ΔCt calculated by subtracting the Ct-value for CD36 to the Ct of the house-keeping gene GAPDH. The fold difference in expression between HuTu80 and GOT1 is shown above the bars. D - F) Replication of sapovirus samples in HuTu80 and GOT1. Left y-axis represents the fold change (FC) of virus at 72 hours post infection (hpi) compared to 2 hpi. The two biological duplicates per infection experiment are shown as black dots. A mean FC of at least 5-fold (dotted line) was considered successful replication and samples with a mean FC of less than 1 were set to 1.1 FC for visualization purposes in the graph. The sample viral load, expressed as genome copies per gram of feces, is shown as colored squares. GAPDH; Glyceraldehyd-3-fosfatdehydrogenas

Article Snippet: Also, since HuTu80 cells originate from EECs, a duodenal adenocarcinoma of L-cell type (ATCC HTB-40), we investigated whether another EEC cell line, GOT1, could serve as a potential model for sapovirus infection.

Techniques: Construct, Immunofluorescence, Staining, Gene Expression, Expressing, Virus, Infection

Journal: bioRxiv

Article Title: Human enterochromaffin cells and apical-out intestinal organoids as models for human sapovirus infection

doi: 10.64898/2026.03.06.710023

Figure Lengend Snippet: Viral load for replicating and non-replicating samples in HuTu80 (left) and GOT1 (right), color coded by genotype. p-value, calculated by Mann-Whitney U test, is displayed. B) Correlation analysis of replication FC and sample viral load in HuTu80 (left) and GOT1 (right), determined by Spearman r. Left y-axis represents the fold change (FC) of virus at 72 hours post infection (hpi) compared to 2hpi. The sample viral load, displayed as genome copies per gram of stool, is shown on x-axis. Error bars show standard deviation in FC between the two biological replicates of the infection experiment. C) Replication of sapovirus GI.1, GII.1 and GII.3 in GOT1 cells in three independent experiments. y-axis shows the fold change (FC) of virus at 72 hours post infection (hpi) compared to 2hpi. The two biological duplicates per infection experiment are shown as black dots. A mean FC of at least 5-fold (dotted line) was considered successful replication. D) Replication kinetics of GI.1, GII.1 and GII.3 in GOT1 cells, presented as a fold change in viral RNA at different time points compared to at 2 hpi. Dotted line represents replication threshold of FC=5.

Article Snippet: Also, since HuTu80 cells originate from EECs, a duodenal adenocarcinoma of L-cell type (ATCC HTB-40), we investigated whether another EEC cell line, GOT1, could serve as a potential model for sapovirus infection.

Techniques: MANN-WHITNEY, Virus, Infection, Standard Deviation

Journal: Genes to Cells

Article Title: Establishment of a Novel Caco‐2‐Based Cell Culture System for Human Sapovirus Propagation

doi: 10.1111/gtc.70007

Figure Lengend Snippet: Susceptibility of various cell lines to HuSaV infection. (A) Changes in HuSaV RNA copy numbers in the culture supernatants of HuTu80 and HEK293T cells following inoculation with a HuSaV GI.1 (AH20)‐positive stool suspension (~4 × 10 7 copies/well in 96‐well plates). Each dot represents individual data points; bars indicate the geometric mean value of the HuSaV RNA copy numbers; error bars denote the geometric standard deviation (SD). This experiment was performed once with five technical replicates. (B) Changes in HuSaV RNA copy numbers in the culture supernatants of Caco‐2, HCT15, HCT116, Caco‐2/Cas9, and C2BBe1 cells following inoculation with a HuSaV GI.1 (AH20)‐positive stool suspension (~2 × 10 6 copies/well in 96‐well plates). Each dot represents individual data points; bars indicate the geometric mean HuSaV RNA copy numbers; error bars denote the geometric SD. This experiment was performed once with five technical replicates. (C) Immunofluorescence staining of the viral protein VP1 in HuTu80, HEK293T, Caco‐2, and Caco‐2/Cas9 cells at 3 dpi with a HuSaV GI.1 (AH20)‐positive stool suspension; upper panels: 4× objective lens, lower panels: 40× objective lens. (D) Flow cytometry analysis of Caco‐2 and Caco‐2/Cas9 cells infected with a HuSaV GI.1 (AH20)‐positive stool suspension at 4 dpi. Blue: VP1‐negative cells (uninfected), red: VP1‐positive cells (infected).

Article Snippet: The human duodenal cancer cell line HuTu80 was purchased from ATCC and cultured in Iscove's Modified Dulbecco's Medium (IMDM; SIGMA) supplemented with 1% GlutaMAX‐I (Gibco, Massachusetts, USA), 1% penicillin/streptomycin (Nacalai Tesque), and 5% heat‐inactivated fetal bovine serum (FBS: CAPRICORN, Ebsdorfergrund, Germany).

Techniques: Infection, Suspension, Standard Deviation, Immunofluorescence, Staining, Flow Cytometry

Journal: Genes to Cells

Article Title: Establishment of a Novel Caco‐2‐Based Cell Culture System for Human Sapovirus Propagation

doi: 10.1111/gtc.70007

Figure Lengend Snippet: Serial passage of HuSaV GI.1 in HuTu80, HEK293T, Caco‐2, and Caco‐2/Cas9 cells. HuSaV RNA copy numbers in the culture supernatants at 0 or 1 dpi (immediately after medium replacement) and 7 dpi for each passage, up to P + 5, in (A) HuTu80, (B) HEK293T, (C) Caco‐2, and (D) Caco‐2/Cas9 cells.

Article Snippet: The human duodenal cancer cell line HuTu80 was purchased from ATCC and cultured in Iscove's Modified Dulbecco's Medium (IMDM; SIGMA) supplemented with 1% GlutaMAX‐I (Gibco, Massachusetts, USA), 1% penicillin/streptomycin (Nacalai Tesque), and 5% heat‐inactivated fetal bovine serum (FBS: CAPRICORN, Ebsdorfergrund, Germany).

Techniques: